Objective: To develop a midwifery career motivation evaluation scale for undergraduate nursing students and to test its reliability and validity, so as to evaluate their willingness to choose a job. Methods: Guided by the framework of expectation-value theory, a midwifery career motivation evaluation scale for undergraduate nursing students was developed by literature review, group discussion and expert consultation. 410 nursing students were selected to test the reliability and validity of the scale. Results: The midwifery career motivation evaluation scale for undergraduate nursing students included expectation subscale and value subscale. The expectation subscale included 5 items, the Cronbach’s alpha coefficients was 0.86 and the retest reliability was 0.69. The value subscale included 18 items, 4 dimensions including practicability, challenge, cost and interest were extracted, and the cumulative variance contribution rate was 68.74%. The Cronbach’s alpha coefficients was 0.93 and the retest reliability was 0.72. Conclusion: The midwifery career motivation evaluation scale for undergraduate nursing students has good reliability and validity, and can be used to evaluate their desire for midwifery choice.