Objective: To analyze the status and influencing factors of maternal-fetal attachment in pregnant women during third trimester. Methods: 210 pregnant women during third trimester who were treated in the obstetrics clinic of a tertiary Class A hospital in Tianjin were selected by convenient sampling from August to December 2019.The general data questionnaire, maternal antenatal attachment scale(MAAS) and other research tools were used to survey.Multiple linear stepwise regression analysis was used for multivariate analysis. Results: The MAAS score of pregnant women during third trimester was 75.93±6.03. Multiple linear stepwise regression analysis showed that per capita monthly income, depression, negative coping and perceived social support were the influencing factors of maternal-fetal attachment( P<0.01).43.3% of the total variance was explained. Conclusion: The level of maternal-fetal attachment of pregnant women during third trimesteris at a high level, which is affected by many factors.Medical staff can take measures to improve the quality of maternal-fetal attachment and promote maternal-fetal health by improving their positive coping ability and social support level.