Objective: To discussion the clinical effect observation of different feeding methods on premature very low birth weight infants (VLBWI). Methods: We retrospectively analysed 323 VLBWI whose gestational age ranged from 26 to 32 weeks and birth weight ranged from 710g to 1500g. According to feeding way, they were divided into human milk fortification group, pure breastfeeding group and artificial feeding group. On the basis of these, we made a statistic analysis on the incidence of necrotizing enterocolitis(NEC) and body length, head circumference, body weight growth rate of infants when the corrected gestational age was 40 weeks. Results: The incidence rate of NEC in human milk fortification group and pure breastfeeding group was lower than that of artificial feeding group. On the incidence rate of NEC, there was no significant difference between human milk fortification group and pure breastfeeding group (P＞0.05). The body length, head circumference, body weight growth rate of infants when the corrected gestational age was 40 weeks, there was no significant statistical differences between human milk fortification group and artificial feeding group (P＞0.05) and they both do much better than pure breastfeeding group. Conclusion: It will not increase the incidence of NEC that taking human milk fortification on premature very low birth weight infants and besides it will reduce the occurrence of postnatal growth retardation.