Objective: To explore the effect of evidence-based practice on prevention of medical device related pressure injury (MDRPI) in critically ill patients based on the Knowledge to Action Cycle. Methods: According to the standard procedure of evidence-based practice in the Knowledge to Action Cycle, the evidence-based practice for the prevention of MDRPI in critically ill patients was formed and applied to clinical effect evaluation. A non-synchronous control trial design was used. Critically ill patients with MDRPI risk admitted from July to September 2019 were included in the control group. Critically ill patients admitted to the same clinical situation from July to September 2020 were included in the intervention group. The control group received routine care and the intervention group received evidence-based practice of prevention on the basis of routine care. The incidence of MDRPI between the intervention group and the control group was compared and the feasibility and effectiveness of the prevention evidence-based practice program in specific clinical situations was evaluated. Results:There was no significant difference in baseline data between the two groups （P>0.05）. The incidence of MDRPI in the intervention group was lower than that in the control group, there was significant statistical difference (P<0.05).Conclusion:Evidence -based practice of MDRPI prevention among critically ill patients was carried out based on the Knowledge to Action Cycle Theory, and a scientific, feasible and evidence-based practice for MDRPI prevention was formed. It reduced the incidence of MDRPI, and improved the quality of skin management among critically ill patients.