Objective: To understand the current situation of nurses’ experience and support as the second victim of adverse nursing events, and analyze the influencing factors. Methods: A total of 421 nurses who had experienced adverse nursing events in a hospital in Tianjin were included by convenience sampling method from January to March 2022. General data questionnaire, second victim experience and support scale and mental toughness scale were used for the survey, and the influencing factors were analyzed by multiple linear regression. Results: The total score of experience and support scale for nurses as the second victim of adverse nursing events was 91.74±12.66, and the total score of mental toughness scale for nurses who had experienced adverse nursing events was 90.12±17.58. There was no correlation between the second victim experience and the total score of support, the second victim total score of pain and mental resilience, and the scores of all dimensions(P>0.05). Organizational support was positively correlated with total score of mental toughness and self-strengthening (P<0.05), while non-job-related support was positively correlated with total score of mental toughness, tenacity, optimism and self-strengthening(P<0.05). Multiple linear regression analysis showed that educational level, professional rank and understanding of the concept of "second victim" were the influencing factors of the pain of nurses as the second victim of adverse nursing events (P<0.05). The number of night shift per month and the total score of mental toughness were influencing factors of organizational support of nurses(P<0.05). Conclusion: The experience and support level of nurses as the second victim of adverse nursing events need to be improved. Adverse nursing events have a negative impact on the physical and mental well-being of nurses as the second victim, and the available support is short. It is suggested that nursing managers should adopt targeted methods to guide and help nurses to alleviate the negative effects of adverse events on nurses’ physical and mental health according to the characteristics of nurses, and improve the experience and support level of nurses as the second victim of adverse nursing events by improving the mental toughness level.