Objective: To investigate the views on the “universal two-child” policy of pregnant women in some regions, and analyze the influence factors on their childbearing desire.Methods: From April to August in 2016, a total of 232 pregnant women from Grade A Class-three hospitals in Beijing, Tianjin, Henan and Jiangsu provinces were surveyed and interviewed by convenient sampling.Results: The awareness rate of the “universal two-child” policy among pregnant women in some regions was high (93.5%). Only 19.8% of the participants support the “universal two-child” policy, 29.3% of the participants had the desire to give birth to a second child, while 62.9% didn’t have the desire. Through interviews with pregnant women in some regions, the main factors influencing their second birth include the cost of birth, career development, and new concept on fertility.Conclusion: The study shows that the participants were mainly born in the 1980s and 1990s, and were at the stage of childbearing age. However, only part of them had the desire to give birth to a second child. The childbearing desire was influenced by factors in many aspects, including macro-factors such as social policy and culture, and micro-factors such as personal and family factors. Therefore, it is necessary to make a comprehensive consideration on the fertility desire when the family planning policy is adjusted, so as to ensure the healthy development of population.