Objective: To evaluate and summarize the best evidence of ocular disinfection before cataract surgery，and to provide evidence support for the prevention of endophthalmitis after cataract surgery. Methods: Chinese biomedical literature database and Joanna Briggs Institute(JBI), National Clinical Guidelines Database(NGC), Australian Clinical Practice Guidelines Database, New Zealand Guidelines Group, Canadian Medical Association CPG Network, UK National Institute for Health and Service Excellence(NICE)，and other databases were retrieved，researches on ocular disinfection before cataract surgery including guidelines, best practice information book, evidence summary and randomized controlled trials were extracted systematically. The retrieval time is from inception to October 24, 2021. Three researchers evaluated the quality of literatures and extracted the contents. Results: A total of 7 guidelines were included. The evidence included the type of disinfectant, the concentration of disinfectant, the scope of disinfection and the time of disinfection. Conclusion:The nursing staff should choose appropriate disinfectant and use correct method to disinfect the eyes before operation, so as to reduce the bacteria community around the eyes and conjunctival sac and prevent the occurrence of endophthalmitis after operation，to ensure the safety of patients. Due to the continuous updating of evidence and humanistic nature of nursing, evidence should be selected pertinently combining with hospital environment and clinical characteristics when the evidences were applied.